聖保祿孝女會主要的敬禮是：道路、真理和生命的耶穌基督、聖保祿及諸宗徒之后聖母瑪利亞。
修會的靈修建基在道路、真理和生命的耶穌基督內，並以聖保祿的生命境界為目標：「我生活已不是我生活，而是基督在我內生活。」（迦二20）
修女們努力效法聖保祿活出基督，並善用現代最有影響力的傳媒，完全為傳播福音而生活。她們生活在諸宗徒之后，聖母瑪利亞的眷顧下，如同聖母把耶穌帶給世界，她們也將耶穌帶給世人。修女們每天除了早禱、晚禱、默想、參與感恩聖祭之外，為聖化自己及人靈，另有一個半鐘頭的朝拜聖體。修女們在耶穌聖體前與耶穌親密交談，讓耶穌改變她們、聖化她們與整個世界，並把世界的痛苦、飢渴與盼望交託給耶穌。
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